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Qualitative and quantitative analysis of the microflora of the gingival sulcus of supporting teeth at the stages of orthopedic treatment

Abstract

Purpose of the study. Conduct a qualitative and quantitative analysis of the microflora of the gingival sulcus of supporting teeth at the stages of prosthetics.

Materials and research methods. For bacteriological examination of the contents of the gingival sulcus of the teeth, sterile endodontic paper points were used, which were inserted into the gingival sulcus affected by inflammation, after which they were placed in a special transport medium Schedler broth (Schadler Broth BBI, USA). In order to determine aerobic and anaerobic bacteria, special nutrient media were used: Columdia agar and Anaerobe Blood agar, Champen Mannitol agar; Saburo Dextrose agar; MRS agar; Shadler agar.

Results of the study and their discussion. The main indicators of qualitative and quantitative analysis in patients with inflammatory lesions of the marginal periodontal tissues of prosthetic origin in the analysis of the composition of the microflora in the gingival sulcus were not detected Streptococcus Anhaemaliticus, Lactobacterium spp., Streptococcus salivaris and Bifidobacterium spp., while in the control group the presence of these bacteria was observed in 12.28%, 7.01%, 3.51% and 7.01% of cases, respectively.

Conclusion. During the study of the bacterial flora isolated from the gingival sulcus of the supporting teeth, it was found that in the patients from the main group observed by us, the contamination of aerobic microorganisms in the gingival sulcus of the supporting teeth was significantly higher than in patients from the control group. Indicators of bacterial contamination by anaerobic pathogens in patients of the main group are lower than in patients from the control group.

About the Authors

G. H. Kurbonova
National Educational Institution “Medical and Social Institute of Tajikistan”
Tajikistan

Kurbonova Gulnoz Khoshimovna - acting Head of the Department of Therapeutic Dentistry,

Tel: (+992) 918 43 43 77.



M. Makhmudov
State Educational Institution “Tajik State Medical University named after A.I. Abuali ibn Sino”
Tajikistan


S. Sattorov
State Educational Institution “Tajik State Medical University named after A.I. Abuali ibn Sino”
Tajikistan


References

1. Abakarov S.I. Colonization of microbial flora in the application of various types of cast fixed prostheses / S.I. Abakarov, V.N. Tsarev, S.E. Umarova // Materials of the XIV and XV All-Russian Scientific and Practical Conferences and Proceedings of the X Congress of the Dental Association of Russia. -M., 2005. -S. 122-124.

2. Bezrukova I.V. Microbiological and immunological aspects of the etiopathogenesis of rapidly progressive periodontitis /I.V. Bezrukova // Periodontology. -2000. -No. 3 (17). -FROM. 3-8.

3. Makhmudov M.M., Satorov S. Inflammatory diseases of the mucous membrane of the prosthetic bed in persons using dentures. /Orthopedic dentistry. - 2017, Vol. 13 No. 1, P. 75-78, Yekaterinburg, USMU

4. Microbial associations of the periodontal pocket in patients with generalized periodontitis /Kosenko K.N. [et al.] //Visnik stomatologii. -Donetsk, 2000. -№ 3. -S. 10-13.

5. Microbial complexes in subgingival plaque /S.S. Socransky [et al.] //J. Clin. Periodontol. –2008. –Vol. 25, N 2. –P. 134144.


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For citations:


Kurbonova G.H., Makhmudov M., Sattorov S. Qualitative and quantitative analysis of the microflora of the gingival sulcus of supporting teeth at the stages of orthopedic treatment. Herald of the medical and social institute of Tajikistan. 2022;(2):5-10. (In Russ.)

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ISSN 3007-3715 (Print)